Fig. 7

Anti-inflammatory property of primary CSSC at P2. Mouse RAW264.7 cells pre-incubated with heat-denatured CM concentrates showed upregulated expression of mouse ACP5, MMP9, and CTSK (blue colored) after RANKL/ConA stimulation for 5 days, similar to the controls (black colored). All three genes were downregulated after treatments using native CM (orange colored) from HC436, 439, 540 and 641, but not with CM from HC515, 534, and 572 (in bracket). The treatment with CM from human stromal fibroblasts (HSF) serves as a negative control without anti-inflammatory effect. Gray bars: naïve RAW cells; dark bars: RAW after RANKL/ConA treatment (without CM). *P < 0.05 and **P < 0.01 compared with RANKL/ConA-treated cells (Mann–Whitney U test)