Fig. 8

Scarring index (SI) represents the anti-scarring potential of donor CSSC. A Distribution of SI (round dots) calculated by our formula revealed donor CSSC (n = 18 cell batches). Labels on the x-axis indicate CSSC from different donors. CSSC with SI < 10 are colored in blue whereas cells with SI > 10 are orange-colored. B Mean percentages of scarring area (squares) inside mouse corneas after treatment with CSSC batches arranged with the same order as in A (n = 8 per group). Most cell treatments resulted in scar reduction, when compared with untreated wound control (100%, thick dark horizontal line), except HC572 and 515 with ineffective outcomes. Treatment with CSSC batches having in vitro SI < 10 (marked by the blue squares). The blue dotted regression line shows a stable regressive line at 32.7 ± 17.4% scar inhibition. For the treatment with CSSC having in vitro SI > 10, the regressive line showed an increasing trend of scar formation (orange dotted line). (C) Representative corneal images showing treatment outcomes with different CSSC having their respective SI values, compared with naïve and untreated wound controls