Good manufacturing practice production of human corneal limbus-derived stromal stem cells and in vitro quality screening for therapeutic inhibition of corneal scarring

Table 1 Comparison of qualified GMP-grade manufacturing reagents to research-grade reagents for key culture steps

	Research protocol	GMP protocol
Limbal tissue digestion	Collagenase A (1 mg/ml; Roche #11,088,793,001)	Collagenase I (Nordmark NB6 GMP, #N0002779)
Culture surface coating	FNC mix (Athena Enzyme #0407)	Recombinant human fibronectin (R&D #4305B-GMP)
Cell detachment	TrypLE Select (Gibco #12,563,011)	TrypLE Select CTS (Gibco #A12859)
Cryopreservation	Dimethyl sulfoxide (DMSO; 5%; Sigma #D2650)	CryoStor®® DMSO (5%; Biolife Solutions Ltd. #210,373)

ISSN: 1757-6512