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Table 2 Culture medium formulation (research versus GMP-grade)

From: Good manufacturing practice production of human corneal limbus-derived stromal stem cells and in vitro quality screening for therapeutic inhibition of corneal scarring

Research-based JM-H medium

Qualified GMP reagents

DMEM, 1 g/L L-glucose (Gibco #10,567–014): MCDB201 (Sigma-Aldrich #M6770) mixed in a 3:2 (vol/vol) ratio

DMEM, 1 g/L L-glucose (Gibco #10,567–014): Ham F12 (Gibco #31,765–035) mixed in a 3:2 (vol/vol) ratio

Antibiotic–antimycotic (1%; Gibco #14,140–122)

Antibiotic–antimycotic (1%; BioWhittaker, Lonza #17-745E)

AlbuMAX I (0.1%; Gibco #11,020–021)

Flexbumin (0.1%; BioSupply #00944–0493-01)

L-ascorbate-2-phosphate (0.5 mM; Sigma-Aldrich #A8960)

L-ascorbic acid (0.5 mM; Tocris Biosci #5778)

Recombinant human EGF (10 ng/mL; Sigma-Aldrich #E9644)

Recombinant human EGF (10 ng/mL; Gibco #PHG6045)

Recombinant human PDGF-BB (10 ng/mL R&D #520-BB)

Recombinant human PDGF (10 ng/mL; R&D #220-GMP-010)

Dexamethasone (10 nM; Sigma-Aldrich #D4902)

Dexamethasone (10 nM; micronized USP, Spectrum #DEE121)

Human serum (2%; Innovative Res #ISER-36670)

Human serum (2%; Innovative Res #ISER-36670)

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Stem Cell Research & Therapy

ISSN: 1757-6512