Fig. 4

Effect of different therapeutic interventions on gene expression linked to RISF. Expression of genes related to inflammation (A–D) (IL-1, IL-6, TNFα, and NF-ĸB) and fibrosis (E–F) (CTGF and TGF-β) was analyzed in the skin of 40Gy irradiated and differentially treated mice: irradiated control, C57BL/6 ASCs, C57BL/6 ASCs + metformin, FVB ASCs, FVB ASCs + metformin, and metformin on day 42 post-irradiation using real-time quantitative PCR (n = 8–10). P value < 0.05 = ^*, < 0.005 = ^**, < 0.0005 = ^***, < 0.00005 = ^****