Fig. 5

Effect of ASCs and metformin on irradiated fibroblasts in transwell co-culture. A–D Mouse fibroblast cell line L929 cells were grown to confluence in the lower chamber of the transwell plate, irradiated with 10Gy irradiation dose, and mouse ASCs, metformin, or both were added 24 h. post-irradiation for 72 h. RNA was isolated from the fibroblasts and analyzed by quantitative RT-PCR for the expression of IL-6 (A), CTGF (B), Col1 (C), and Col3 (D). E–H Human foreskin fibroblasts were grown to confluence in the lower chamber of the transwell plate, irradiated with 10Gy irradiation dose, and human ASCs, metformin, or both were added 24 h post-irradiation for 72 h. RNA was isolated from the fibroblasts and analyzed by quantitative RT-PCR for the expression of IL-6 (E), CTGF (F), Col1 (G), and Col3 (H). P value < 0.05 = ^*, < 0.005 = ^**, < 0.0005 = ^***, < 0.00005 = ^****