Fig. 3
Pulp regeneration after complete disinfection with levofloxacin and nanobubbles in model B. A–C, E, F, I Hematoxylin and eosin staining. A, B Regenerated pulp tissue. C, D Odontoblastic cells (arrows) lining to newly formed osteodentin/tubular dentin (OD) along with the dentin. D Immunohistological staining for Dspp. E Osteodentin (OD) was regenerated in the coronal part of the root canal. F Periapical tissue healing. Alveolar bone (AB). Periodontal ligament (PdL). G Blood vessels stained by BS-1 lectin (arrows). H Neuronal process stained by PGP 9.5 (arrows). I Healing of external resorption (arrow) with formation of cementum (Ce). Periodontal ligament (PdL). J, K Modification of the Brown and Brenn Gram staining. Gram-negative bacteria (arrows). J Cementum (Ce). K Periapical tissue (Pa)