Fig. 4

Organoid-derived intestinal epithelial cells have polarity A Immunocytochemistry of RYU on LONG with the antibody to Vimentin and CDX2. RYU and LONG are positive for CDX2 and Vimentin, respectively. Scale bar: 100 µm. B Transmission electron microscopic analysis of RYU on LONG. Adhesion between RYU and LONG was observed. Scale bars: 1000 (left) and 500 nm. C Histology and immunohistochemistry of RYU in iPGell. Immunohistochemistry was performed with antibodies to CDX2, AE1/AE3, and Vimentin. Scale bars: 50 µm. D Immunohistochemistry of RYU in iPGell was performed with antibodies to Villin, BCRP, P-gp, and PEPT1. Strongly stained areas are indicated by black arrows. Scale bars: 50 µm. E Alcian Blue stain of RYU in iPGell. Scale bars: 50 and 20 (inset) µm. F Immunohistochemistry of RYU in iPGell with antibodies to MUC2. Scale bar: 50 µm. G Transmission electron microscopic analysis of epithelial apical membrane side. Microvilli and glycocalyx (yellow squares) were observed. Scale bar: 200 nm. H Scanning electron microscopic analysis of RYU on LONG. Microvilli were observed over the surface of RYU. Scale bar: 500 nm. I Schematic of RYU on LONG. RYU are polarized in adherent culture. The apical and basal sides of RYU are in contact with culture medium and LONG, respectively