Fig. 10

hGC β-galactosidase staining (100 ×). With the extension of H₂O₂ treatment time, the cells gradually became senescent, β-galactosidase activity gradually increased, and the blue staining rate gradually increased. Compared with 48 h, although the cells were more in line with the senescence condition at 72 h, the number of remaining cells was small compared with the 48-h condition. Therefore, 200 μmol/L H₂O₂ was selected to treat hGCs for 48 h for follow-up experiments. a β-galactosidase staining of hGC after 24h H₂O₂ treatment b β-galactosidase staining of hGC after 48h H₂O₂ treatment c β-galactosidase staining of hGC after 72h H₂O₂ treatment d Statistical graph of hGC senescence rate