Fig. 2
Effect of ASC-Ectos and ASC-Exos on the proliferation, migration, and chondrogenesis of BMSCs. PBS treatment served as a control. (a) Representative fluorescence micrograph of EV uptake by BMSCs. The blue and red colors represent the nucleus and PKH26-labeled EVs, respectively. (b) BMSC viability measured by CCK-8 at 24, 48, and 72 h after co-incubation with EVs. (c) Representative images showing the transwell migration assay and scratch assay of BMSCs treated with EVs for 24 and 48 h. (d) Quantitative analysis of the migrated BMSCs. (e) Quantitative analysis of the wound closure. (f) Representative micrograph of BMSC chondrogenic microspheres. (g) Representative light micrograph (left) and Alcian blue staining (right) of BMSC chondrogenic microspheres. (h) Quantitative analysis of chondrogenic microsphere pellet area. (i) Gene expression level of collagen II and aggrecan detected by qPCR. (j) Protein expression level of collagen II and aggrecan detected by WB. Scale bar: 100 μm. *p < 0.05. Data were presented as mean ± SD of three replicates