Fig. 1

Nuclear movement generated cellular protrusions that appeared and disappeared from the surface of hBM-MSC-derived intermediate cells. A Time-lapse imaging showed that when hBM-MSCs were exposed to neural induction medium, they rapidly reshaped from a flat to a spherical morphology. Subsequently, we observed hBM-MSC-derived intermediate cells (white asterisk) in which nuclear movements generate only one cell protrusion (white arrow) and hBM-MSC-derived intermediate cells (yellow asterisk) in which nuclear movements alternately generate one or two cellular protrusions (yellow arrows). B We found that when an hBM-MSC-derived intermediate cell has a nucleus without lobes, its movement within the cell generates only one cell protrusion (yellow arrow). C However, if the hBM-MSC-derived intermediate cell has a lobed nucleus, it will generate one or two cellular protrusions depending on how it moves within the cell (yellow arrows). Scale bar: 10 μm. PhC: Phase-contrast photomicrographs. The number at the top indicates the time since the time-lapse image began. Elapsed time is displayed in the format (hours:minutes)