Fig. 2

Characterization of hBM-MSC-derived intermediate cell nuclei. A Immunocytochemical analysis revealed that hBM-MSC-derived intermediate cells primarily have three different nuclear morphologies: Tail-less nuclei (white asterisk), tailed nuclei (green asterisk) and lobed nuclei (yellow asterisk). Tail-less and tailed nuclei movements generate only one cell protrusion (white and green arrows, respectively). However, lobed nuclei movements generate one or two cellular protrusions depending on how they move within the cell (yellow arrows). Confocal microscopy analysis and 3D reconstruction revealed that there are small variations in both shape and size within the three types of nuclear morphology (B) and that the lobes of the lobed nuclei can be located in different z-planes. C Immunocytochemical analysis revealed that tail-less nuclei and tailed nuclei contained one or two fibrillarin-positive specks, while lobed nuclei contained one or two fibrillarin-positive specks in each lobe. D The inner surface of the nuclear envelope is lined by the nuclear lamina (E). Scale bar: 10 μm. PhC: Phase-contrast photomicrographs