Fig. 3
Cytoskeletal organization of hBM-MSC-derived intermediate cells. A Immunocytochemical analysis revealed that actin microfilaments and β-III tubulin microtubules are no longer oriented parallel to the longitudinal axis of the hBM-MSC-derived intermediate cells. B Confocal microscopy analysis and 3D reconstruction revealed that cell protrusions are almost devoid of actin microfilaments and that the β-III tubulin protein is concentrated at the cell protrusion rim (black arrows). Scale bar: 10 μm