Fig. 6

Lobed nuclei formation by two nuclear remodelling sequences. Time-lapse imaging showed that first, a nuclear remodelling sequence occurs as the cell reshapes from a flat to a spherical morphology, positioning the nucleus in a peripheral position within the cell (white arrows). Subsequently, the cell nucleus moves (green arrows) and undergoes a second nuclear sequence in which a lobed nucleus is formed (yellow arrows). Afterwards, the lobed nuclei began to move within the hBM-MSC-derived intermediate cells (green arrows). Finally, we also noted that lobed nuclei can switch shape while moving within the cell, becoming tailed nuclei (blue arrows). Scale bar: 10 μm. The number at the top indicates the time since the time-lapse image began. Elapsed time is displayed in the format (hours:minutes)