Fig. 4

Characterization of the cells during maintenance culture (A) and in vitro expansion ability (B) and self-renewal capacity (C) of mouse ABSCs. A During the subculture process, cells in the colonies are p63/Krt5 double-positive cells (scale bar, 200 μm). B, C Comparisons of the PDT (B, n = 3) and CFE (C, n = 6) of mouse ABSCs at passage 5, 7, 10, 13, and 15. Mouse ABSCs maintain stable in vitro expansion and self-renewal capacity, and the cell cultures from P10 demonstrated the shortest doubling time and the highest colony-forming capacity. The comparison between groups was determined using one-way analysis of variance (ANOVA) followed by the Tukey multiple comparison test. *P < 0.05. **P < 0.01. ***P < 0.001