Fig. 5
From: Culture and expansion of murine proximal airway basal stem cells
In vitro differentiation culture of mouse ABSCs. A Morphological manifestations of cells during in vitro differentiation culture. B–D The expression of p63/Krt5 (B), Act-Tub (C), MUC5AC (C), PDPN (D), and SPC (D) in vitro differentiation culture. During in vitro differentiation culture, mouse ABSCs could maintain the self-renewal of p63/Krt5 double-positive cells (B), differentiate into both Act-Tub-positive ciliated cells and MUC5AC-positive mucus-secreting cells (C), and not differentiate into both PDPN-positive alveolar type I cells and SPC-positive alveolar type II cells (E) (scale bar, 100 μm)