Fig. 6
From: Culture and expansion of murine proximal airway basal stem cells
Three-dimensional tracheospheres culture of mouse ABSCs. A Brightfield images shows formation of tracheospheres. B H and E staining of tracheospheres (scale bar, 50 μm). C-E. Using IHF assay to detect the p63/Krt5 (C), Act-Tub (D), MUC5AC (D), PDPN (E), and SPC (E) expression in tracheospheres generated in 3D culture. During 3D tracheosphere culture, mouse ABSCs could maintain the self-renewal of p63/Krt5 double-positive cells (C), differentiate into both Act-Tub-positive ciliated cells and MUC5AC-positive mucus-secreting cells (D), and not differentiate into both PDPN-positive alveolar type I cells and SPC-positive alveolar type II cells (E) (scale bar, 50 μm)