Fig. 1From: Amelioration of diabetic nephropathy in mice by a single intravenous injection of human mesenchymal stromal cells at early and later disease stages is associated with restoration of autophagyDesigns of In vivo Experiments Diagram summarizing the designs of two experiments for investigating the effects of hUC-MSCs on kidney disease in the C57BL/6 mouse model of streptozotocin (STZ)-induced diabetes mellitus. As shown, mice were injected intra-peritoneally (i.p.) with 80 mg/kg STZ (diabetic/DM) or citrate buffer (nondiabetic/Non-DM) for 5 consecutive days. One week later, DM was confirmed by fasting blood glucose (FBG) > 16.7 mM. Eight weeks and 16 weeks after the injection of STZ/citrate buffer, groups of 6 DM and non-DM mice received 0.2 mL intravenous injections of normal saline (NS) or 5 × 105 human umbilical cord-derived mesenchymal stromal cells (hUC-MSCs) as illustrated. Two weeks later (10 weeks or 18 weeks following initiation of STZ/citrate buffer injections), mice were euthanized and urine, serum and kidneys were collected for analysesBack to article page