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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Amelioration of diabetic nephropathy in mice by a single intravenous injection of human mesenchymal stromal cells at early and later disease stages is associated with restoration of autophagy

Fig. 2

Differentiation and Surface Marker Profile of hUC-MSCs: a–c Representative photomicrographs demonstrating a Oil red O staining of lipid droplets in hUC-MSCs under adipogenic culture conditions, b Alizarin red staining of hUC-MSCs under osteogenic culture conditions and c Alcian blue staining of hUC-MSC pellets cultured under chondrogenic conditions. Scale bar, 100 μm. d Representative flow cytometry dot plot demonstrating hUC-MSC scatter characteristics and histograms demonstrating relative fluorescence following surface staining with monoclonal antibodies against: CD90, CD73, CD45, CD29, CD44, CD105 and HLA-DR. Differentiation and surface marker expression experiments were repeated in triplicate

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