Fig. 5

Kidney histology and ultrastructural analyses from two in vivo experiments. a Representative images of H&E, PAS, and Masson’s trichrome stained kidney sections from Non-DM, DM + NS and DM + MSC mice, analyzed 10 and 18 weeks after induction of DM [magnification: 400× (Scale bar 36  μm) and 200× (Scale bar 76  μm)]. b Graphical representation of quantification of Mesangial index (% glomerular area) and tubulointerstitial fibrosis of Non-DM, DM + NS and DM + MSC mice at 10 and 18 weeks after induction of DM. c Representative examples of transmission electron microscopy images of glomeruli of individual animals from each of experimental groups demonstrating ultrastructural features of the GBM and podocytes (Scale bar 2 μm). Arrowhead represents foot processes of the podocytes; asterisk represents GBM. d Graphical representation of quantification of thickness of GBM. For graphs, data are presented as mean ± SEM. *p < 0.05 versus Non-DM group. ^†p < 0.05 versus DM + NS group