Fig. 4
TC14012 enhances the anti-fibrotic effect of UC-MSCs. The CCl4-induced liver fibrotic mice were treated with UC-MSCs with or without TC14012 pretreatment. a The morphological liver was observed. b Liver function was evaluated by examined the serum levels of AST, each group n = 6 mice. c and e The accumulation of collagen fibers in liver were performed by Sirius Red staining (c) and Masson trichrome staining (e). Scale bar: 500 μm. g The activation of HSCs were analyzed in liver by immunofluorescent staining of α-SMA. Scale bar: 200 μm. d, f, h The quantitative analysis of Sirius Red staining (d), Masson trichrome staining (f), and α-SMA staining (h), each group n = 6 mice. The data were presented as the mean ± SD. One-way ANOVA and Student’s t-test: **p < 0.01, ***p < 0.001, ****p < 0.0001