Fig. 5

Identifying the transcriptomic characteristics of H1-KO, H2-KO and H1/H2-dKO cardiomyocytes. A Schematic diagram of the samples for RNA-seq. WT, H1-KO, H2-KO and H1/H2-dKO cardiomyocytes were collected at differentiation day 7. B PCA analysis of WT, H1-KO, H2-KO and H1/H2-dKO cardiomyocytes. Each group had three duplicates. C The DEGs between different groups. DEGs were defined with |fold change (FC)|> 2 and adjusted P-value < 0.05. D The expression of representative FHF, SHF and cardiomyocyte (CM) genes in RNA-seq. E Gene expression cluster analysis of RNA-seq. Genes included in clustering were at least differentially expressed in one comparison in (C). F GO enrichment analysis of each gene expression cluster. G KEGG pathway enrichment analysis of each gene expression cluster