Fig. 3

Primary culture, immunofluorescence and flow cytometry identification of rLESCs. a Representative images (40×) of rLESCs migration on day 2, 4, and 6. The blue arrows indicate the edge of the tissue block. The red arrows indicate the edge of the migrated rLESCs. b Representative images of cytokeratin 3/12-stained CECs and rLESCs (left) and the corresponding positive rates in CECs and rLESCs (right). Scale bars = 100 μm. c Representative flow cytometrical detection of deltaNP63 expression (left) and the corresponding positive rates (right) in CECs and rLESCs. The isotype control images for the rLESCs group are presented in Additional file 1: Figure S1. Data is presented as the mean ± SD for each group from the three experiments. ***p < 0.001