Fig. 4

Cytocompatibility of 4D-CTH cell carriers. a After 4D-CTH carrier was dried, the transferred cells were observed by electron microscope. The red arrows indicate the rLESCs adhered to the scaffold and partially distributed within the mesh. b CCK-8 analysis of rLESC-enriched limbal epithelium seeded in the normal culture medium and scaffold material (n = 3). c Representative images (left) and quantitative analysis of the wound healing rate (right) of the scratch area in the control group and scaffold material group at 24 and 48 h. d Representative live/dead images (left) and quantification of cell viability (right) in the normal culture medium and scaffold material after 24, 48, and 72 h of culturing. The data is expressed as mean ± SD **p < 0.01; ***p < 0.001, n ≥ 3