Fig. 1
Cell acquisition and safety assessment after cell transplantation: iPSCs were cultured in PSC neural induction medium on a Geltrex-coated 6-well plate for 7 days, and iPSCs differentiated into NSCs. After 13 days, the cells were passaged as the first NSC generation, and the cells were cultured for another four to six days to obtain the second passage NSC. The third passage NSC was selected for the experiment. Human umbilical cords were cut and uniformly fixed at the bottom of the Petri dish, and cultured in an incubator at 37 °C with 5% CO2 after adding MSC medium. The passage was observed after 14 days, and the fourth passage was selected for the experiment. The final number of MSC and NSC cells was mixed 1:1 before treatment (A). B Spleen weights of mice were recorded after sacrifice. C Spleen weight-body weight ratio of mice was recorded after sacrifice. D Recordings of body weight weighing performed every other day after cell transplantation. E Size plot of mouse spleens after sacrifice