Fig. 2
The sequential recruitment of neutrophils and monocytes into the injured site post-CI. A Representative images for pan-CD45 + (leukocytes) cells at specified time points post-CI. The dotted line represents the boundary of the injured site. B Representative IHC staining images of Ly6G + cells at the indicated time intervals. C Quantitation of Ly6G+ cells fields are shown. Ly6G+ cells were quantified in 5 random fields by quantifying positive cells per sample, n = 3/24 per time point. The data were averaged and presented as mean ± SD. **P < 0.01, ****p < 0.0001. D mRNA expression of Ly6G at the injured liver as compared to sham control. E Representative images for CD11b+ immunostaining showing monocyte trafficking at the injured site at the indicated time intervals. F Quantitation of CD11b+ cells per 5 fields is shown. CD11b+ cells are quantified by quantifying positive pixels in 5 random fields, averaged, and presented as mean ± SD of the positive area. n = 3/24 per time point. *p < 0.05, **p < 0.01, ****p < 0.0001. Scale bars 100 µm. Arrows (in B and E) indicate the infiltration of neutrophils (Ly6G+) or monocytes (CD11b+) at 3 h