Fig. 4

Depletion of Kupffer cells (KCs) substantially reduces the recruitment of monocytes from blood. A Experimental design of intravenous injection of either PBS liposomes (PLs) or Clodronate liposomes (CLs), induction of CI procedures and samples collection at specified time intervals. B Representative images of depletion of KCs shown in CLs-treated samples in comparison to PLs-treated and sham sections. KCs after depletion began to reappear at the injury site at day 6 post-CI. C Effect of KCs depletion on monocytes in PLs/CLs-treated samples as compared to sham samples. CLs treatment delayed the recruitment of monocytes, scale bar 100 µm, n = 3/27 per time point