Fig. 5

KCs hindrance affects the translocation of allo-HPs in the sinusoids. A Experimental design: CI was induced at day 0 (D0), and GFP tag allo-HPs were engrafted 48 h after induction of CI in wild-type C57 mice. Samples were subsequently collected 54 h post-engraftment. B Representative images of sham-treated liver samples post-transplantation are shown. Scale bars 100 µm (inset = 50 µm). C Transplanted allo-HPs were localized in the portal vein (PV) at the non-injured site. Scale bars 100 µm (inset = 50 µm). D Very few allo-HPs were detected at the vicinity of the injured site. E KCs dismantle allo-HPs in the sinusoids (inset = 50 µm, non-injured site). The arrows indicate KCs invading the clusters of allo-HPs. F Injured site representing KCs (F4/80⁺) cells. Scale bar 100 µm (inset = 50 µm), n = 3/27