Fig. 1

MG gliosis fate determination occurred at 3–5 days after sodium iodate-induced retinal acute injury. a Immunohistochemical labeling for GFAP, tdTomato and DAPI in the central retinas of control and SI-treated groups. b Relative fluorescent intensity GFAP protein in control and SI-treated retinas over time (n = 5). c, d Western blot analysis of GFAP protein expression in control and SI-treated retinas (n = 4), full-length gels and blots are presented in Additional file 1: Fig. S8. e Representative whole-mount retina partial images of tdTomato and EdU staining. Insets display the enlarged drawings of each fluorescent reporter (White Square). White solid arrows identify the tdTomato and EdU double positive cells. f Ratio of EdU and tdTomato double positive cells in each whole-mount retina of control and SI-treated groups (n = 6). g Representative retinal section partial images of tdTomato, EdU and DAPI staining. Insets display the enlarged drawings of each fluorescent reporter in EdU and tdTomato (White Square). White arrows identify the EdU and tdTomato double positive cells. h Ratio of EdU and tdTomato double positive cells in the ONL of control and SI-treated retinas (n = 6). i Ratio of migrated MG in control and SI-treated retinas (n = 6). ONL Outer nuclear layer, INL Inner nuclear layer, GCL Ganglion cell layer. *P < 0.05; **P < 0.01; ***P < 0.001, one-way ANOVA test was used in (b, d, f, h, i)