Fig. 8
From: The m6A methylation and expression profiles of mouse neural stem cells after hypoxia/reoxygenation
Knockdown of Mettl14 inhibited the proliferation of NSCs after H/R. A Detection of the cell viability of NSCs that undergo H/R treatment after Mettl14 knockdown by CCK-8 assay (n = 3). **P < 0.01; ***P < 0.001. B The EdU-positive cells in NSCs treated with H/R after Mettl14 knockdown were observed by immunofluorescence staining (n = 3). Scale bar = 40 μm. **P < 0.01; ***P < 0.001. C NSCs in suspended and adherent states. D The cell spheroidizing ability of NSCs. E Detection of the geometric mean diameter of neurosphere that undergo H/R treatment after Mettl14 knockdown by cell spheroidization assay (n = 3). **P < 0.01; ***P < 0.001. The data are presented as the mean ± SEM