Fig. 3

LRP1^high is the subpopulation with high secretory function. A UMAP feature plot shows the expression levels of genes related to the function regulated by LRP1 in human UC-MSCs. B The expression levels of LRP1, secreted factors and extracellular matrix related genes in different subpopulations were detected by qRT-PCR. Mean ± SD, ** P ≤ 0.01; * P ≤ 0.05. C The secretion levels of CXCL8 and IL1β in LRP1^high and LRP1^low subpopulations and unsorted human UC-MSCs were detected by ELISA. The expression levels of these two factors were highest in LRP1^high subpopulation and lowest in LRP1^low subpopulation. Mean ± SD, ** P ≤ 0.01; * P ≤ 0.05; ns: > 0.05. D qRT-PCR was used to detect the expression levels of LRP1 and secretory factors in each subpopulation of human UC-MSCs on the 3rd and 6th days after sorting. The sorted human UC-MSCs subgroups were able to maintain their functionality for a period of time. Mean ± SD, n = 3, **** P ≤ 0.0001; *** P ≤ 0.001; ** P ≤ 0.01; * P ≤ 0.05