Fig. 4
The LRP1high subpopulation significantly restored ovarian function in aged mice and CTX-induced POF mice. A Changes of the serum hormone levels in aged mice after human UC-MSCs transplantation. Compared with LRP1low subpopulation and unsorted human UC-MSCs, LRP1high subpopulation can significantly restore serum LH, E2 and AMH levels in aged mice. The number of mice used was represented by different shaped dots in each group. Mean ± SD, **** P ≤ 0.0001; *** P ≤ 0.001; ** P ≤ 0.01; * P ≤ 0.05; ns: > 0.05. B Changes in estrous cycle of young mice, aged mice, and subpopulation treated aged mice. The entire estrous cycle was monitored for a duration of three weeks. The day of initial detection was labeled as "1," and each subsequent stage change was denoted by " + 1." If the stage remained the same as the previous day, the number from the previous day was retained, and this pattern continued for the full 21 days. C The total number of complete estrous cycles experienced by the mice within the three-week period was recorded, and the average duration of the estrous cycle was calculated for each group of mice. The number of mice used was represented by different shaped dots in each group. Mean ± SD, *** P ≤ 0.001; ** P ≤ 0.01; * P ≤ 0.05. D The H&E staining of the ovaries in CTX-induced POF mice after transplantation of subpopulations. E Statistical graph showing the changes in the number of ovarian follicles within the ovaries in D. The number of mice used was represented by different shaped dots in each group. F Quantification of E12.5 embryos in CTX-induced POF mice after transplantation of subpopulations. The number of mice used was represented by different shaped dots in each group