Fig. 5
From: Oxidized SOD1 accelerates cellular senescence in neural stem cells
Exogenous EVs from senescent NSCs induce aging-like phenotypes. A. Internalization of exogenous EVs. Shown are representative immunofluorescence staining of misfolded hSOD1 (B8H10, Green) and hSOD1 (blue) after treatment with EVsWT and EVsG93A from P5 NSCs. EVs were labeled with Texas Red. Scale bar: 2 μm. B. Immunofluorescence staining of P3 NSCsWT with HMGB1 (Green) and Nestin (Red) after treatment with P5 EVsWT and EVsG93A. Senescent NSCs were detected by cytoplasmic translocation of HMGB1 and by expressing SA-β-gal. Scale bar: 10 μm. C. The translocation of HMGB1 from the nucleus to the cytosol in the P3 recipient NSCsWT. D. Quantification of SA-β-gal positive cells. For C and D, one-way ANOVA followed by Bonferroni’s Multiple Comparison Post-Test was performed. The experiments were conducted in triplicate from three independent cultures