Fig. 2
From: Short cell cycle duration is a phenotype of human epidermal stem cells
Expanding colonies show predominantly PP divisions over 12 generations in vitro, while differentiating colonies show an increased likelihood of DD divisions at subsequent generations. (A) Proportion of division types (PP, PD, DD) in expanding and differentiating colonies. (B) Mean proportion of P and D cells produced in expanding colonies over generations in vitro. One-way ANOVA, P = 0.09. (C) Plating freshly obtained cells results in keratinocytes with a range of differentiation status. (D) Mean proportion of P and D cells produced in differentiating colonies, examined from the terminal differentiation division backwards. One-way ANOVA, P < 0.0001. (E) Mean proportion of P and D cells produced in expanding colonies over time, examined from the final observed division backwards. One-way ANOVA, P = 0.57. Data presented as mean ± SEM. (): colonies analyzed. TD: terminal differentiation. FO: final observation. EXP: expanding colonies. DIF: differentiating colonies