Fig. 6
Adenosine induces iPericyte relaxation. A, B Normalised cell index of neural crest iPericytes, mesoderm iPericytes and HBVPs treated with adenosine or vehicle (CPM) over a period of 2 h (n = 4 per condition). C–E Quantified AUC (C), ∆ cell index (D) and ∆ cell index after 2 h (E) for mesoderm iPericytes and HBVPs treated with control or adenosine analysed using two-way ANOVA: AUC (cell type: F (1, 12) = 6.583, p = 0.0247; treatment: (F (1, 12) = 26.84, p = 0.0002; interaction: F (1, 12) = 6.027, p = 0.0303); ∆ cell index (cell type: F (1, 12) = 6.387, p = 0.0265; treatment: F (1, 12) = 28.26, p = 0.0002; interaction: F (1, 12) = 1.284, p = 0.2794); ∆ cell index after 2 h (cell type: F (1, 12) = 1.460, p = 0.2502; treatment: F (1, 12) = 0.1232, p = 0.7317; interaction: F (1, 12) = 1.174, p = 0.2999). F–H Quantified AUC (F), ∆ cell index (G) and ∆ cell index after 2 h (H) for neural crest iPericytes and HBVPs treated with control or adenosine analysed using two-way ANOVA: AUC (cell type: F (1, 12) = 8.596, p = 0.0126; treatment: (F (1, 12) = 50.38, p < 0.0001; interaction: F (1, 12) = 7.159, p = 0.0202); ∆ cell index (cell type: F (1, 12) = 7.881, p = 0.0158; treatment: F (1, 12) = 57.12, p < 0.0001; interaction: F (1, 12) = 3.777, p = 0.0758); ∆ cell index after 2 h (cell type: F (1, 12) = 16.46, p = 0.0016; treatment: F (1, 12) = 20.58, p = 0.0007; interaction: F (1, 12) = 1.500, p = 0.2442). C–H Post-hoc comparisons performed using Sidak’s multiple comparisons test. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. Data shown as mean ± SD. I, J Normalised gene expression counts of differentially expressed adenosine receptors in HBVP, neural crest iPericytes and mesoderm iPericytes compared using DEseq: HBVPs and neural crest iPericytes ADORA1, I log2FoldChange = 3.92, padj = 1.65E−11; ADORA2B, J log2FoldChange = − 1.71, padj = 2.93E−19; HBVPs and mesoderm iPericytes ADORA1, I log2FoldChange = 2.72, padj = 0.00006; ADORA2B, J log2FoldChange = − 2.33, padj = 1.52E−25; neural crest iPericytes compared to mesoderm iPericytes ADORA1, I log2FoldChange = − 1.19, padj = 0.001; ADORA2B, J log2FoldChange = − 0.61, padj = 0.03288