Fig. 3

The bioink shows good biocompatibility and integration on rat ex vivo cornea (n = 2 rats including total of 4 corneas) and human DM (n = 6 donors). hPSC-CEnCs differentiated from the used hESC line. A, B Representative flat mount immunofluorescence stainings of hPSC-CEnCs in bioink injected on rat ex vivo cornea (n = 2) after 5 days of injection and C, D hPSC-CEnCs seeded without bioink on rat ex vivo cornea (n = 2) after 5 days of seeding. E Representative IgG controls of hPSC-CEnCs seeded without bioink on rat ex vivo cornea. F Representative images of hPSC-CEnCs in bioink injected on human DM (n = 3) and G) hPSC-CEnCs seeded without bioink on human DM (n = 3) after 6 days of injection/seeding. In A, C) ZO-1 (green) STEM121 (red) and Hoechst (cyan); B, D) Na⁺/K⁺-ATPase (green) CD166 (red) and Hoechst (cyan); E) IgG control for Alexa Fluor 488 (green), IgG control for Alexa Fluor 568 (red) and Hoechst (cyan); F–G ZO-1 (green) CD166 (red) and Hoechst (cyan). Scale bars 50 µm (A–E), 20 µm (F–G)