Fig. 4

The effects of bioink on hPSC-CEnC cell size, barrier and Ca²⁺-signaling properties after 6 days of injection/seeding. Experiments conducted with the hESC line. A Cell number analysis between bioink injected cells and seeded cells with representative ROI-images (n = 6 per sample type) from which the cell number calculations were measured. B TEER value measurements of the hPSC-CEnCs injected with bioink, seeded without bioink (n = 4 per sample type) and bioink without cells (n = 3). TEER value of empty insert (n = 4) was subtracted from the values. All samples were treated with identical medium changes. C Cell number of responding hPSC-CEnCs injected in bioink and seeded without bioink (ctrl). D Relative percent of responding hPSC-CEnCs injected in bioink and seeded without bioink (ctrl). All data represents mean ± SD. A) **p = 0.002, B) *p < 0.05, no significant difference between other samples, C–D no significant difference was detected. ROI-image size 200 × 200 pixels taken with 25 × objective. Scale bars 20 µm (A)