Fig. 6

Immunofluorescence stainings of monolayered bioprinted hPSC-CEnCs on fibrin membrane and the patternability of the bioink. Experiment conducted with the hESC line. A Representative confocal microscope images of cryosections stained with ZO-1, CD166, Na⁺/K⁺-ATPase and Hoechst 7 days after printing on fibrin membrane (n = 1). B Representative images of hPSC-CEnCs printed in a line pattern with a 1.3 mm line distance on cell culture plastic after 1 day (n = 2). ZO-1 (green), Phalloidin (purple) and Hoechst (nuclei, blue). Scale bars 50 µm (A), 500 µm (B)