Fig. 6

The effects of pro-inflammatory (a), hypoxic (b), and 3D culture priming (c)on the transcriptome of MSCs were semi-quantified by qPCR at three time points: day 0 (directly following 48 h of priming), day 4, and day 8. Fold changes are shown and represent the gene expression levels of primed MSCs from three healthy donors compared to the gene expression levels of unprimed MSCs at time point day 0. Lines connect the mean fold changes of the three healthy donors. The significance levels for the gene expression differences between each time point and condition, compared to the gene expression directly after priming at day 0 are shown. Additionally, for each time point separately, significant gene expression differences between primed MSCs cultured under standard conditions and primed MSCs cultured under translationally relevant conditions are highlighted by significance indicators (not significant: p > 0.05; *: p ≤ 0.05; **: p ≤ 0.01; ***: p ≤ 0.001)