Fig. 6

UC/PL-MSC-CM inhibits Rho/MRTF/SRF signaling in HIMFs. HIMFs were treated with TGF-β1 (5 ng/mL) and co-cultured with or without UC/PL-MSC-CM. A RT-qPCR analysis of the relative mRNA expression of MRTFA, SRF, RHOA, ROCK1, and ROCK2. The data were normalized to GAPDH expression and expressed as relative values compared to the control (n = 3). B Representative western blots show the protein expression of MRTF-A, SRF, and RhoA (MRTF-A and SRF from the nuclear extracts with HDAC1 as a loading control and RhoA from the cytosolic extracts with GAPDH as a loading control). C Quantitation of MRTF-A, SRF, and RhoA from western blot analyses (n = 3). Data are expressed as the means ± SEM. ^##P < .01 and ^###P < .001 versus the control; ^*P < .05, ^**P < .01 and ^***P < .001 versus the TGF-β1 treatment only (ANOVA w/ Tukey); ^†P < .05 compared between PL-MSC-CM and UC-MSC-CM (Mann–Whitney U test). NS, not significant. Full-length blots are presented in Additional file 1: Fig. S4