Fig. 5

The LSM model distinguishes HSPCs sorted with LSK/CD34/CD135 surface markers and α-catulin-GFP. A Representative FACS density dot plots show the gating strategy employed to sort murine LT-HSCs, ST-HSCs, and MPPs using LSK/CD34/CD135 surface markers. B The performance metrics of the LSM model in the classification of HSPCs obtained from A. A total of 4142 LT-HSCs, 873 ST-HSCs, and 1780 MPPs were obtained from 4 C57BL/6 mice and tested. C DIC and fluorescence images of LSK/α-catulin-GFP⁺ cells were taken immediately after FACS. Representative images are shown. Scale bar = 10 μm. D Total 1227 LSK/α-catulin-GFP⁺ cells were obtained from 4 α-catulin^GFP mice and analyzed by the LSM model. 74% of them were classified as LT-HSC