Fig. 1

Characteristics of fMSCs and aMSCs. Representative morphologic images of (A) fMSCs and (B) aMSCs, respectively. (C) CASY TT measurement of the mean peak cell diameter of fMSCs and aMSCs (n = 3). (D) The mean number of population doublings (PD) that fMSCs and aMSCs achieved per passage between passage 5–8, and (E) The mean population doubling time (PDT) in hours for fMSCs and aMSCs over passage 5–8. Representative images of β-galactosidase expression in (F) fMSCs and (G) aMSCs at passage 8. The arrowheads indicate β-galactosidase positive cells (blue), n = 3. (H) The mean percentage of β-galactosidase positive cells in fMSCs and aMSCs at passage 8 (n = 3). (I) The mean colony forming unit fibroblast (CFU-F) capacity of fMSCs between passage 3–8, (adult MSCs did not have CFU-F capacity after passage 4), n = 3. Mean ± SD, *P < 0.05, **P < 0.01. Scale bars in (A, B, F, G) represents 200 μm