Fig. 2

Differentiation of fMSCs and aMSCs into osteoblasts and adipocytes. Representative images of the whole 12-plate wells following Alizarin red S staining of calcium deposits in osteogenic induced MSCs; (A) fMSCs and (B) aMSCs. Representative microscopic images at 100× magnification of osteogenic induced and Alizarin red S stained (extracellular red staining) (C) fMSCs and (D) aMSCs. (E) Quantification of Alizarin red S staining of osteogenic differentiated and control fMSCs and aMSCs. Representative microscopic images at 100× magnification of (F) fMSCs and (G) aMSCs induced into adipocytes following Oil Red O staining (red intracellular lipid droplets). (H) Quantification of Oil Red O staining of adipogenic differentiated and control fMSCs and aMSCs. Two replicate experiments for each donor, n = 3 for fMSCs and aMSCs. Mean ± SD, *P < 0.05, **<0.01, ns = not significant