Fig. 4

Effect of human embryonic stem cell-derived mesenchymal progenitor cells (hESC-MPCs) on ovulation, embryonic development, pregnancy and litter sizes in perimenopausal mice. (A) Ovulation rate, calculated as the number of ovulated mice per total mice. (B) The average number of eggs recovered per mouse. (C) Representative images of embryos after 120 h of culture in vitro. Scale bars = 50 μm. (D) Percentage of blastocyst formation after in vitro fertilization. (E-H) Female mice from the young group and experimental group were individually caged with a young male for mating. (E) Percentage of plug-positive mice per total mice. (F) Percentage of mothers that delivered viable fetuses per mouse. (G) Average number of offspring per mouse. (H) Average number of offspring per litter. Three-month-old (young), 14-month-old (control), and 14-month-old mice treated monthly for 4 months with hESC-MPCs (hESC-MPCs). Different superscript letters indicate significant differences (p < 0.05)