Fig. 3
Differences in morphology and protein expression between ELC-org and ELC-org-mono. (A) Phase-contrast images of ELC-org and ELC-org-mono are shown. (B) TEM images of ELC-org and ELC-org-mono are shown. Microvilli (black arrows) and tight junctions (black arrowheads) are indicated. (C) Percentages of EpCAM- and Villin-positive cells in ELC-org and ELC-org-mono were measured by FACS analysis. Negative control (blue) and stained cells (red) are represented. (D) The protein expressions of VIL, CHGA, ECAD and LYZ in ELC-org were characterized by immunostaining. (E) The protein expression of ZO-1 in ELC-org-mono was characterized by immunostaining. (F) The protein expressions of CYP3A4, VIL, CHGA, ECAD, LYZ, CDX2 and MUC2 in ELC-org-mono were characterized by immunostaining