Fig. 2

Optimal concentration of ASPP 049 increases hiPSC-derived HSPCs in vitro. A Cell viability of hiPSC-derived nonadherent cells is relative to DMSO vehicle control and B CD34⁺CD45⁺ fractions represented as the percentage of flow cytometry gating in the presence of various concentrations (0.1, 1, and 10 μM) of ASPP 049. A and B Individual data are represented on bar charts with mean ± SEM error bars from three biological replicates. **p < 0.01 compared with DMSO (repeated-measures one-way ANOVA with Dunnett's multiple comparisons). C Representative flow cytometry analysis of hiPSC-derived nonadherent cells harvested on day 12, assessed by phenotypically defined markers of HSPCs (CD34⁺CD45⁺) and primitive HSPCs (CD34⁺CD45⁺CD90⁺). D Fold change analysis of the hematopoietic phenotypes of derived cells relative to hiPSC-seeded colonies in the presence of 1 μM ASPP 049. Individual data relative to DMSO are represented on a bar chart with mean ± SEM error bars from three biological replicates. *p < 0.05, **p < 0.01 compared with DMSO (paired student’s t-test)