Fig. 3

ASPP 049 modulates the developmental endothelial progenitors through endothelial-to-hematopoietic transition (EHT). A Kinetics of flow cytometry analysis of ASPP 049 (1 μM) on EHT defined by the expression of endothelial progenitor markers (CD144 and CD73) and hematopoietic markers (CD34 and CD45). The developmental endothelial potency of adherent cells: hemogenic endothelial progenitors (HEP, CD144⁺CD73⁻), vascular endothelial progenitors (VEPs, CD144⁺CD73⁺), and hematopoietic committed cells (CD34⁺CD45⁺) during hematopoietic differentiation are shown. B–D Statistical analysis of each endothelial progenitor number of different time points relative to hiPSC-seeded colonies. Individual data are represented on a bar chart with mean ± SEM error bars from three biological replicates. *p < 0.05, **p < 0.01 compared with DMSO vehicle control simultaneously (paired student's t-test)