Fig. 5
The effect of ASPP 049 on gene expression profiles of the Hippo signaling pathway. A Unsupervised heatmap was obtained with sample cluster analysis of gene expression profiles in hiPSC-derived HSPCs (hiPS-HSPC) with or without ASPP 049, n = 4. B Volcano plot represents the differentially expressed genes (DEGs) between hiPS-HSPC and ASPP 049 treated hiPSC-derived HSPCs (hiPS-HSPC + ASPP 049), mean fold change (log2) > 1, p-value significance (− log10) > 1.4. C Gene set enrichment ratio of significant pathways coordinated with transcriptomic alteration in 46 DEGs in hiPS-HSPC and hiPS-HSPC + ASPP 049. D Heatmap of the protein-coding genes relates to the Hippo signaling pathway induced in hiPS-HSPC + ASPP 049: no significantly different genes (black label) and significantly different genes (red label). E Bar chart of gene set enrichment categorically analyzed in Biological Process, Cellular Component, and Molecular Function. Individual data of expression levels is represented from four biological replicates as a color ranging of mRNA expression levels: blue, downregulated; black, no difference; yellow, upregulated