Fig. 6

Validation of Hippo signaling-related gene expression profiles in hiPSC-derived HSPCs. A Using quantitative real-time PCR compared with NanoString® mRNA analysis, the expression of ASPP 049-associated top significant genes is represented as mean ± SEM of the fold change (log₂) relative to vehicle control from two biological replicates (each technical triplicates) B Immunoblotting analysis of hiPSC-derived CD34⁺ cells on day 12 hematopoietic differentiation with and without ASPP 049 supplementation probed with anti-YAP and anti-TAZ in both active (no phosphorylation) and inactive (phosphorylation) forms. Full-length blots are presented in Additional file 1: Fig. S4. C Immunofluorescence analysis of protein expression levels and localizations of these Hippo signals influenced by the effect of ASPP 049 (1 μM) on hiPSC-derived CD34⁺ cells compared to vehicle control from two biological replicates. D Quantitative real-time PCR of ASPP 049-associated Hippo-targeted genes represents mean ± SEM of the fold change (log₂) relative to vehicle control from two biological replicates (each technical triplicate)