Fig. 7

ASPP 049 supplementation induced gene expression patterns correlating with stemness similar to UCB-derived CD34⁺ cells (CD34⁺ UCB). A Venn diagram of three different gene lists between key DEGs of hiPS-HSPC vs. hiPS-HSPC + ASPP 049 (gene set 1, orange), non-DEGs of hiPS-HSPC vs. CD34⁺ UCB (gene set 2, blue), and non-DEGs of hiPS-HSPC + ASPP 049 vs. CD34⁺ UCB (gene set 3, red) identified a subset of 11 rescued genes in response to closely correlated patterns of CD34⁺ UCB. B Heatmap showing the protein-coding gene expressions of hiPS-HSPC + ASPP 049 (green) were rescued (not upregulated or down-regulated) in similar to CD34⁺ UCB (red) compared with hiPS-HSPC (dark blue). C and D Heatmap of Hippo signaling-related genes C and RhoROCK signaling-related genes D showing the induction of hiPS-HSPC + ASPP 049 related to the rescued patterns compared with hiPS-HSPC and CD34⁺ UCB. Individual data of expression levels is represented from four biological replicates as a color ranging of mRNA expression levels: blue, downregulated; black, no difference; yellow, upregulated